Harbor-UCLA Pediatrics

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Tsui-Fen Chou, Ph.D.

Tsiu-Fen Chou, PhD

Tsui-Fen Chou, Ph.D.
Assistant Professor of Pediatrics
Division of Medical Genetics

Contact Information:
Child Health Research Center
1124 W. Carson St.
Torrance, CA 90502

Phone: (424) 201-3006
Email: tchou@labiomed.org

Research Focus:
Dr. Chou's research interests include chemical biology, cancer cell biology, medicinal chemistry, biochemistry, quantitative proteomics, yeast genetics, and high-throughput screening. Her main research focus is on discovering underlying mechanisms that may lead to new therapeutic targets for cancer.

Postdoctoral fellowship, Proteomics, California Institute of Technology, Pasadena, CA

Ph.D., Medicinal Chemistry, University of Minnesota, Twin Cities, Minneapolis, MN
Minor: Biochemistry, Molecular Biology and Biophysics
B.S., Pharmacy, School of Pharmacy, National Taiwan University, Taipei, Taiwan

Selected Publications:

1. Chou, T.-F., and Wagner, C. R. “Lysyl-tRNA synthetase generated lysyl-adenylate is a substrate for histidine triad nucleotide binding proteins (Hints).” J. Biol. Chem. 2007, 282, 4719 - 4727.
2. Chou, T.-F., Sham, Y. Y., and Wagner, C. R. “The Impact of the C-terminal loop of histidine triad nucleotide binding protein1 (Hint1) on substrate specificity.” Biochemistry, 2007, 46, 13074 - 13079.
3. Chou, T.-F., So, C., White, B.R., Carlson, J.C.T., Sarikaya, M., and Wagner, C.R. “Enzyme nanorings” ACS Nano 2008, 2, 2519 - 2525.
4. Chou, T.-F.*, and Deshaies, R. J*. “Quantitative cell-based protein degradation assays to identify and classify drugs that target the ubiquitin-proteasome system. J. Biol. Chem. 2011, 286, 16546 - 16554. *Co-corresponding authors
5. Chou, T.-F.*, Brown, S. J., Minond, D., Nordin, B.E., Li, K., Jones, A.C., Chase, P., Porubsky, P. R., Stoltz, B.M., Schoenen, F. J., Patricelli, M.P., Hodder, P., Rosen, H., and Deshaies, R. J*. “Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways” Proc. Natl. Acad. Sci. USA. 2011, 108, 4834 - 4839. *Co-corresponding authors
6. Chou, T.-F*., and Deshaies, R. J*. “Development of p97 AAA ATPase inhibitors. Autophagy. 2011, 7, 1091-1092. *Co-corresponding authors
7. Chou, T.-F*., Li, K., Frankowski, K., Schoenen, F. J., and Deshaies, R. J*. “Structure-Activity Relationship study reveals ML240 and ML241 as potent and selective inhibitors of p97 ATPase” ChemMedChem. 2013, 8,297-312. *Co-corresponding authors
8. Zhou, X., Chou, T.-F, Aubol, B.E., Park, C. J.,Wolfenden, R., Adams, J. and Wagner, C.R. “Kinetic Mechanism of Human Histidine Triad Nucleotide Binding Protein 1 (Hint1) ” Biochemistry, 2013, 52, 3588-3600
9. Chou, T.-F*, Bulfer, S. L., Weihl, C.C., Li, K., Lis, L. G., Walters, M. A., Schoenen, F.J., Lin, H. J., Deshaies, R. J, and Arkin, M. R. “Specific Inhibition of p97/VCP ATPase and Kinetic Analysis Demonstrate Interaction between D1 and D2 ATPase domains” J. Mol. Biol. 2014, 426, 2886-2899. *Corresponding author
10.Gonzalez, M.A., Feely, S., Speziani, F., Strickland, A., Danzi, M., Bacon, C., Youjin Lee, Y., Chou, T.-F, Blanton, S.H., Weihl, C.C., Zuchner, S., Shy, M. “A Novel Mutation in VCP Causes Charcot-Marie-Tooth Type 2 Disease” Brain, 2014, PMID: 25125609.
11. Sapir, A., Tsur, A., Koorman, T., Ching, K., Bardenheier, A., Podolsky, L., Bening-Abu-Shach, U., Boxem, M., Chou, T.-F, Broday, L., and Sternberg, P.W. “Controlled sumoylation of the mevalonate pathway enzyme HMGS-1 regulates metabolism during aging” Proc. Natl. Acad. Sci. USA. 2014, 111, E3880-3889.
12. Fang, C., Gui, L., Zhang, X., Moen, D.R., Li, K., Frankowski, K. J., Lin, H.J., Schoene, F.J., and Chou, T.-F “Evaluating p97 inhibitor analogues for their domain-selectivity and potency against the p97-p47 complex” ChemMedChem, 2015, 10, 52-56. (PMID: 25377500)
13. Kato, M., Chou, T.-F, Yu, C.Z., Demodena, J., and Sternberg, P.W. “"LINKIN, a new transmembrane protein necessary for cell adhesion” eLife. 2014, e04449. (PMID:25437307)
14. Weihl, C. C., Baloh, R. H., Lee, Y., Chou, T.-F, Pittman, S. K., Lopate, G., Allred, P., Jockel-Balsarotti, J., Pestronk , A., and Harms, M. B. “Targeted sequencing and identification of genetic variants in sporadic inclusion body myositis. Neuromuscul Disord., 2015, Jan 6. pii: S09608966(14)00730-5. doi: 10.1016/j.nmd.2014.12.009. [Epub ahead of print]
15. Zhang, X., Gui, L., Zhang, X., Bulfer, S. L., Sanghez, V., Wong, D., Lee, Y.J., Lehmann, L., Lee, J. S., Shih, P.-Y., Lin, H. J., Iacovino, M., Weihl, C. C., Arkin, M. R., Wang, T., Chou, T.-F. (2015) Altered cofactor regulation with disease associated p97/VCP mutations. Proc. Natl. Acad. Sci. USA, Accepted

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