Harbor-UCLA Pediatrics

eLearning Login

Tsui-Fen Chou, Ph.D.

Tsiu-Fen Chou, PhD

Tsui-Fen Chou, Ph.D.
Assistant Professor of Pediatrics
Division of Medical Genetics

Contact Information:
Child Health Research Center
1124 W. Carson St.
Torrance, CA 90502

Phone: (424) 201-3006
Email: tchou@labiomed.org

Research Focus:
Dr. Chou's research interests include chemical biology, cancer cell biology, medicinal chemistry, biochemistry, quantitative proteomics, yeast genetics, and high-throughput screening. Her main research focus is on discovering underlying mechanisms that may lead to new therapeutic targets for cancer.

Training:
Postdoctoral fellowship, Proteomics, California Institute of Technology, Pasadena, CA

Education:
Ph.D., Medicinal Chemistry, University of Minnesota, Twin Cities, Minneapolis, MN
Minor: Biochemistry, Molecular Biology and Biophysics
B.S., Pharmacy, School of Pharmacy, National Taiwan University, Taipei, Taiwan

Selected Publications:

1. Chou, T.-F., and Wagner, C. R. “Lysyl-tRNA synthetase generated lysyl-adenylate is a substrate for histidine triad nucleotide binding proteins (Hints).” J. Biol. Chem. 2007, 282, 4719 - 4727.
2. Chou, T.-F., Sham, Y. Y., and Wagner, C. R. “The Impact of the C-terminal loop of histidine triad nucleotide binding protein1 (Hint1) on substrate specificity.” Biochemistry, 2007, 46, 13074 - 13079.
3. Chou, T.-F., So, C., White, B.R., Carlson, J.C.T., Sarikaya, M., and Wagner, C.R. “Enzyme nanorings” ACS Nano 2008, 2, 2519 - 2525.
4. Chou, T.-F.*, and Deshaies, R. J*. “Quantitative cell-based protein degradation assays to identify and classify drugs that target the ubiquitin-proteasome system. J. Biol. Chem. 2011, 286, 16546 - 16554. *Co-corresponding authors
5. Chou, T.-F.*, Brown, S. J., Minond, D., Nordin, B.E., Li, K., Jones, A.C., Chase, P., Porubsky, P. R., Stoltz, B.M., Schoenen, F. J., Patricelli, M.P., Hodder, P., Rosen, H., and Deshaies, R. J*. “Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways” Proc. Natl. Acad. Sci. USA. 2011, 108, 4834 - 4839. *Co-corresponding authors
6. Chou, T.-F*., and Deshaies, R. J*. “Development of p97 AAA ATPase inhibitors. Autophagy. 2011, 7, 1091-1092. *Co-corresponding authors
7. Chou, T.-F*., Li, K., Frankowski, K., Schoenen, F. J., and Deshaies, R. J*. “Structure-Activity Relationship study reveals ML240 and ML241 as potent and selective inhibitors of p97 ATPase” ChemMedChem. 2013, 8,297-312. *Co-corresponding authors
8. Zhou, X., Chou, T.-F, Aubol, B.E., Park, C. J.,Wolfenden, R., Adams, J. and Wagner, C.R. “Kinetic Mechanism of Human Histidine Triad Nucleotide Binding Protein 1 (Hint1) ” Biochemistry, 2013, 52, 3588-3600
9. Chou, T.-F*, Bulfer, S. L., Weihl, C.C., Li, K., Lis, L. G., Walters, M. A., Schoenen, F.J., Lin, H. J., Deshaies, R. J, and Arkin, M. R. “Specific Inhibition of p97/VCP ATPase and Kinetic Analysis Demonstrate Interaction between D1 and D2 ATPase domains” J. Mol. Biol. 2014, 426, 2886-2899. *Corresponding author
10.Gonzalez, M.A., Feely, S., Speziani, F., Strickland, A., Danzi, M., Bacon, C., Youjin Lee, Y., Chou, T.-F, Blanton, S.H., Weihl, C.C., Zuchner, S., Shy, M. “A Novel Mutation in VCP Causes Charcot-Marie-Tooth Type 2 Disease” Brain, 2014, PMID: 25125609.
11. Sapir, A., Tsur, A., Koorman, T., Ching, K., Bardenheier, A., Podolsky, L., Bening-Abu-Shach, U., Boxem, M., Chou, T.-F, Broday, L., and Sternberg, P.W. “Controlled sumoylation of the mevalonate pathway enzyme HMGS-1 regulates metabolism during aging” Proc. Natl. Acad. Sci. USA. 2014, 111, E3880-3889.
12. Fang, C., Gui, L., Zhang, X., Moen, D.R., Li, K., Frankowski, K. J., Lin, H.J., Schoene, F.J., and Chou, T.-F “Evaluating p97 inhibitor analogues for their domain-selectivity and potency against the p97-p47 complex” ChemMedChem, 2015, 10, 52-56. (PMID: 25377500)
13. Kato, M., Chou, T.-F, Yu, C.Z., Demodena, J., and Sternberg, P.W. “"LINKIN, a new transmembrane protein necessary for cell adhesion” eLife. 2014, e04449. (PMID:25437307)
14. Weihl, C. C., Baloh, R. H., Lee, Y., Chou, T.-F, Pittman, S. K., Lopate, G., Allred, P., Jockel-Balsarotti, J., Pestronk , A., and Harms, M. B. “Targeted sequencing and identification of genetic variants in sporadic inclusion body myositis. Neuromuscul Disord., 2015, Jan 6. pii: S09608966(14)00730-5. doi: 10.1016/j.nmd.2014.12.009. [Epub ahead of print]
15. Zhang, X., Gui, L., Zhang, X., Bulfer, S. L., Sanghez, V., Wong, D., Lee, Y.J., Lehmann, L., Lee, J. S., Shih, P.-Y., Lin, H. J., Iacovino, M., Weihl, C. C., Arkin, M. R., Wang, T., Chou, T.-F. (2015) Altered cofactor regulation with disease associated p97/VCP mutations. Proc. Natl. Acad. Sci. USA, Accepted

Pubmed Search:
PubMed Search for Tsui-Fen Chou